Hormone & Urinary Metabolites Assessment Profile (Doctor's Data)
The Hormone and Urinary Metabolites Assessment Profile (HuMap) provides a comprehensive overview of hormones and their metabolites, as well as efficiency of enzymes involved in their metabolism.
The Hormone and Urinary Metabolites Assessment Profile (HuMap) provides a comprehensive overview of steroid hormones, their metabolites, and the efficiency of the enzymes that metabolize these hormones. This non-invasive test requires only 4 or 5 separate urine collections. Because the breakdown of hormones relies so heavily on processes within the liver, this test can also help reveal patterns of liver metabolism and conjugation. Additionally, testing urinary hormone metabolites can contribute to further understanding of endogenous hormone secretion, supplemental hormone utilization, enzyme activity, oxidative stress, and insight into how your body metabolizes hormones.
11-Deoxycortisol (male)
Optimal range: 0.3 - 1.2 ng/mg Creat/Day11-hydroxy-Androsterone (male)
Optimal range: 210 - 920 ng/mg Creat/Day
11-Hydroxy-Androsterone (OHAN) is a urinary metabolite of cortisol metabolism as well as 11-oxygenated androgens production from the adrenal glands. While research is limited in the significance of elevations of this metabolite, it may be associated with certain conditions like 21-hydroxylase deficiency and castration- resistant prostate cancer.
LEARN MORE11-hydroxy-Etiocholanolone (male)
Optimal range: 35 - 380 ng/mg Creat16-OH-E1 % (male)
Optimal range: 10 - 50 %16a-Hydroxyestrone (male)
Optimal range: 0.47 - 4.9 ng/mg Creat/Day17-Hydroxyprogesterone (male)
Optimal range: 0.19 - 0.85 ng/mg Creat/Day2-Hydroxyestradiol (male)
Optimal range: 0.018 - 0.55 ng/mg Creat/Day2-Hydroxyestrone (male)
Optimal range: 2.68 - 8.6 ng/mg Creat/Day2-M-E1:2-OH-E1 (male)
Optimal range: 0.06 - 0.32 Ratio2-M-E2:2-OH-E2 (male)
Optimal range: 0.03 - 0.26 Ratio2-Methoxyestradiol (male)
Optimal range: 0.01 - 0.08 ng/mg Creat/Day2-Methoxyestrone (male)
Optimal range: 0.5 - 1.6 ng/mg Creat/Day2-OH-E1 % (male)
Optimal range: 40 - 88 %2-OH-E1:16-OH-E1 (male)
Optimal range: 1.5 - 7.9 Ratio21-Hydroxyprogesterone (male)
Optimal range: 0.6 - 3 ng/mg Creat/Day
21-Hydroxyprogesterone is a steroid hormone with mineralocorticoid properties produced in the adrenal gland which serves as a precursor hormone to aldosterone.
LEARN MORE4-Hydroxyestradiol (male)
Optimal range: 0 - 0.5 ng/mg Creat/Day
4-OH-E2 is associated with a higher risk of certain cancers and other negative markers for breast health in females. Due to the lack of research in this area, it may be postulated that males carry a similar risk.
LEARN MORE4-Hydroxyestrone (male)
Optimal range: 0 - 0.5 ng/mg Creat/Day
Higher levels indicate slowed COMT activity (methylation) and are associated with a higher risk for breast cancer in females. Due to the lack of research in this area, it may be postulated that men carry a similar risk. Elevation may also be due to an overactive CYP1B1 enzyme or sluggish CYP1A1 or CYP34A. Additional support for the COMT enzyme can help with the conversion toward the inactive
metabolite, 4-M-E1.
4-M-E1:4-OH-E1 (male)
Optimal range: 0.04 - 0.5 Ratio4-M-E2:4-OH-E2 (male)
Optimal range: 0.01 - 0.5 Ratio4-Methoxyestradiol (male)
Optimal range: 0.013 - 0.034 ng/mg Creat/Day
Methyl metabolites are considered inactive and are correlated with antiproliferative effects. Proper elimination of 4-M-E2 requires optimal excretion via GI tract optimization. To fully understand this value, it may be beneficial to examine the 4-M-E2 / 4-OH-E2 ratio.
LEARN MORE4-Methoxyestrone (male)
Optimal range: 0.031 - 0.17 ng/mg Creat/Day4-OH-E1 % (male)
Optimal range: 2 - 10 %4-OH-E1:2-OH-E1 (male)
Optimal range: 0 - 0.14 Ratio5-pregnenetriol (male)
Optimal range: 35 - 105 ng/mg Creat/Day
Pregnenetriol is a metabolite of 17a-pregnenolone, an intermediary resulting from the hydroxylation of pregnenolone by CYP17A1 enzyme.
LEARN MORE5a-Androstanediol (male)
Optimal range: 10 - 108 ng/mg Creat/Day5a-Dihydrotestosterone (male)
Optimal range: 0.7 - 6.3 ng/mg Creat/Day5A-PD:5B-PD (male)
Optimal range: 0.06 - 0.24 Ratio
The metabolic prioritization for alpha or beta reductase activity within the progesterone pathway may be confirmatory of a general preference of metabolism. Comparing these results with the metabolic preference of androgens and corticoids may provide additional insight.
LEARN MORE5a-Pregnanediol (male)
Optimal range: 9 - 50 ng/mg Creat/Day
The most important progesterone metabolite, pregnanediol (PDL), can serve as a urinary marker for endogenous progesterone levels and as an indicator of ovulation. PDL exists as two isomers, 5α-pregnanediol and 5β-pregnanediol. 5β-pregnanediol represents the majority end point of endogenous progesterone metabolism and appears to have little activity within the body, while 5α-pregnanediol, the lesser metabolite of PDL, can cross the blood brain barrier and may partially agonize GABA-A receptors.
LEARN MORE5a-Tetrahydrocorticosterone (male)
Optimal range: 90 - 380 ng/mg Creat/Day5a-Tetrahydrocortisol (male)
Optimal range: 420 - 1060 ng/mg Creat/Day5A-THF+5B-THF/THE (male)
Optimal range: 0.6 - 1.2 Ratio5A-THF/5B-THF ratio (male)
Optimal range: 0.15 - 0.65 Ratio5b-Androstanediol (male)
Optimal range: 18 - 200 ng/mg Creat/Day5b-Pregnanediol (male)
Optimal range: 55 - 250 ng/mg Creat/Day
The most important progesterone metabolite, pregnanediol (PDL), can serve as a urinary marker for endogenous progesterone levels and as an indicator of ovulation. PDL exists as two isomers, 5α-pregnanediol and 5β-pregnanediol. 5β-pregnanediol represents the majority end point of endogenous progesterone metabolism and appears to have little activity within the body, while 5α-pregnanediol, the lesser metabolite of PDL, can cross the blood brain barrier and may partially agonize GABA-A receptors.
LEARN MORE5b-Tetrahydrocorticosterone (male)
Optimal range: 58 - 240 ng/mg Creat/Day
5B-THB is a terminal metabolite of corticosterone. This metabolite in combination with other terminal metabolites can be used to estimate metabolism of corticosterone. While research in elevations or low levels of single terminal metabolites is limited, assessing metabolism may provide valuable information about enzyme activity.
LEARN MORE5b-Tetrahydrocortisol (male)
Optimal range: 690 - 2240 ng/mg Creat/Day8-hydroxy-2’-deoxyguanosine (male)
Optimal range: 0 - 7.7 ng/mg Creat/DayAllopregnanolone (male)
Optimal range: 0.8 - 6.4 ng/mg Creat/Day
Allopregnanolone is a potent neuroactive steroid capable of binding the GABA-A receptor often leading to sedative and anxiolytic action. The calming action of allopregnanolone is often seen with orally supplemented progesterone, as the liver metabolizes a large portion of oral progesterone to the neuroactive steroid allopregnanolone.
LEARN MOREAndrostenedione (Male)
Optimal range: 0.8 - 7.7 ng/mg Creat/DayAndrosterone (5a) / Etiocholanolone (5b) (male)
Optimal range: 0.5 - 1.4 RatioAndrosterone (male)
Optimal range: 470 - 2400 ng/mg Creat/DayCorticosterone (male)
Optimal range: 6 - 34 ng/mg Creat/DayCortisol Bedtime (male)
Optimal range: 2 - 10 ng/mg Creat
Cortisol is the main glucocorticoid released from the adrenal gland in response to stress. High levels of cortisol have been reported in cases of Cushing’s disease, malnutrition, early life stress, hypothyroidism, depression, alcoholism, obesity, and critical illness. Additionally, exogenous exposure to glucocorticoids prior to testing may be a source of cortisol elevations.
LEARN MORECortisol Dinnertime (male)
Optimal range: 3 - 18 ng/mg CreatCortisol Waking (male)
Optimal range: 6 - 40 ng/mg Creat
Cortisol is the main glucocorticoid released from the adrenal gland in response to stress. High levels of cortisol have been reported in cases of Cushing’s disease, malnutrition, early life stress, hypothyroidism, depression, alcoholism, obesity, and critical illness. Additionally, exogenous exposure to glucocorticoids prior to testing may be a source of cortisol elevations.
LEARN MORECortisol Waking+2hrs (male)
Optimal range: 14 - 110 ng/mg Creat
Cortisol is the main glucocorticoid released from the adrenal gland in response to stress. High levels of cortisol have been reported in cases of Cushing’s disease, malnutrition, early life stress, hypothyroidism, depression, alcoholism, obesity, and critical illness. Additionally, exogenous exposure to glucocorticoids prior to testing may be a source of cortisol elevations.
LEARN MORECortisol/Cortisone (male)
Optimal range: 0.18 - 0.6 Ratio
Cortisol / cortisone ratio indicates activity of HSD11B2 activity and assessment of tissue specific concentrations of cortisol, which normally cannot be measured without a biopsy.
LEARN MORECortisol/day (male)
Optimal range: 9 - 35 ng/mg Creat/Day
Cortisol is the main glucocorticoid released from the adrenal gland in response to stress. High levels of cortisol have been reported in cases of Cushing’s disease, malnutrition, early life stress, hypothyroidism, depression, alcoholism, obesity, and critical illness. Additionally, exogenous exposure to glucocorticoids prior to testing may be a source of cortisol elevations.
LEARN MORECortisone Bedtime (male)
Optimal range: 10 - 55 ng/mg Creat
Cortisone is the inactive form of cortisol. Elevations of cortisone may reflect high cortisol production, excessive 11BHSD2 activity, or insufficient conversion by 11BHSD1.
LEARN MORECortisone Dinnertime (male)
Optimal range: 15 - 100 ng/mg CreatCortisone Waking (male)
Optimal range: 25 - 95 ng/mg CreatCortisone Waking+2hrs (male)
Optimal range: 45 - 280 ng/mg CreatCortisone/day (male)
Optimal range: 30 - 95 ng/mg Creat/DayCreatinine Bedtime (male)
Optimal range: 35 - 240 mg/dLCreatinine Dinnertime (male)
Optimal range: 35 - 240 mg/dLCreatinine Waking (male)
Optimal range: 35 - 240 mg/dLCreatinine Waking+2hrs (male)
Optimal range: 35 - 240 mg/dLCreatinine/day (male)
Optimal range: 35 - 240 mg/dL/DayDehydroepiandrosterone (male)
Optimal range: 18 - 170 ng/mg Creat/Day
Dehydroepiandrosterone (DHEA) is predominantly produced in the adrenal glands and serves as a precursor hormone for androstenedione and eventually estrone and testosterone. High levels of DHEA may be due to DHEA or pregnenolone supplementation. Additional research suggests DHEA elevations may also be due to such conditions as adult-onset adrenal hyperplasia, congenital adrenal hyperplasia, and very rarely, adrenal carcinoma. SULT2A1 catalyzes the sulfate conjugation of DHEA, and research suggests dopamine can induce this enzyme.
LEARN MOREDehydroepiandrosterone Sulfate (male)
Optimal range: 25 - 660 ng/mg Creat/Day
Dehydroepiandrosterone sulfate or DHEA-S is the sulfated form of dehydroepiandrosterone (DHEA) and the major steroid precursor in humans. This sulfation is reversibly catalyzed by sulfotransferase 2A1 (SULT2A1) primarily in the adrenals, the liver, and the small intestine.
LEARN MOREDHEA+DHEAS (male)
Optimal range: 39 - 762 ng/mg Creat/Day
DHEA and DHEAs are produced in the adrenal gland and serve as precursors to androgens and estrogens. Due to the interconversion between DHEA and DHEAS via SULT2A1 and/or STS, the sum of these maybe a better representation of total DHEA synthesis.
LEARN MOREEPI-Testosterone (male)
Optimal range: 0 - 40 ng/mg Creat/DayEstradiol (male)
Optimal range: 0.4 - 2 ng/mg Creat/Day
Estradiol level is most consistent with exogenous exposure, supplementation, or aromatization of testosterone to estradiol. CYP19, also known as aromatase, can be upregulated raising intracellular estrogens in men which can contribute to increased adiposity, metabolic syndrome, and prostate pathology. CYP19 enzyme is induced during times of stress, exposure to xeno-estrogens, high glycemic diet, excessive adipose tissue, and alcohol consumption.
LEARN MOREEstriol (male)
Optimal range: 1.2 - 4.1 ng/mg Creat/DayEstrone (male)
Optimal range: 1.8 - 5 ng/mg Creat/Day
A component of the estrone level may be due to aromatization of androstenedione and testosterone by CYP19 (aromatase) enzyme in adipose tissue, and/or conversion from estradiol due to HSD17B activity. Aromatase up-regulation and increased intracellular estrogens in men may contribute to increased adiposity, metabolic syndrome, and prostate pathology. CYP19 enzyme is induced during times of stress, exposure to xeno-estrogens, high glycemic diet, excessive adipose tissue, and alcohol consumption.
LEARN MOREEtiocholanolone (male)
Optimal range: 490 - 2100 ng/mg Creat/DayProgesterone (male)
Optimal range: 0 - 0.34 ng/mg Creat/Day
Progesterone is produced by the corpus luteum following ovulation and to a lesser extent by the adrenal glands in both sexes. While found in the urine in small amounts, progesterone can be seen as a clinical marker of luteal activity and theraputic oral progesterone administration.
LEARN MORETestosterone (male)
Optimal range: 12 - 63 ng/mg Creat/DayTestosterone / EPI-Testosterone (male)
Optimal range: 0.6 - 2.4 RatioTetrahydrocortisone (male)
Optimal range: 1200 - 3000 ng/mg Creat/Day
Tetrahydrocortisone is a terminal metabolite of cortisone. This metabolite along with the other terminal metabolites can be used to determine metabolized cortisol. Research in elevations or low levels of single terminal metabolites is limited, assessing metabolized cortisol and daily output may provide more clinically more relevant information.
LEARN MORETetrahydrodehydrocorticosterone (male)
Optimal range: 44 - 150 ng/mg Creat/DayTHE+5A-THF+5B-THF (male)
Optimal range: 2000 - 6000 ng/mg Creat/Day
This calculation includes the daily metabolites of cortisol (5-alpha THF, THF) and cortisone (THE) which maybe a better representation of daily cortisol output than measuring cortisol and cortisone alone due to metabolism differences in the liver (with thyroid hormone) and fatty tissues.
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