Vitamin B9 (Folate)

Other names: FOLATE, SERUM, FOLIC ACID, Folate (Folic Acid), Serum, FOLATE,SERUM, S.Fol.

check icon Optimal Result: 3 - 20 ng/mL, 6.8 - 45.32 nmol/L, or 3 - 20 ug/L.

What is Folate and Why is it Important?

Folate, a vital B vitamin (B9), is essential for various bodily functions, including the production of red blood cells, white blood cells, platelets, and supporting normal cell growth. This nutrient is crucial for fetal development, playing a key role in forming a healthy brain and spinal cord in the early stages of pregnancy. Since the body cannot produce folate on its own, it must be obtained through foods like citrus fruits, dark leafy greens, whole grains, beans, B-vitamin-enriched cereals, as well as kidney and liver.

Why Might You Need a Folate Test?

A healthcare provider may order a folate test for various reasons, including:

- Identifying the cause of anemia (low red blood cell count)

- Checking for malnutrition or malabsorption issues (e.g., celiac disease, inflammatory bowel disease)

- Monitoring treatment for folate deficiency

- Ensuring pregnant individuals have sufficient folic acid to help prevent birth defects

Folate's Role in Pregnancy and Fetal Health

Adequate folate levels are critical in the first few weeks of pregnancy, as low folate can prevent the neural tube from closing properly, leading to neural tube defects, such as:

- Spina bifida: incomplete development of the spinal cord or vertebrae

- Anencephaly: incomplete development of major parts of the brain

To reduce the risk of these defects, expecting mothers are recommended to consume 400 micrograms of folate daily, especially during the early stages of pregnancy when the neural tube is developing.

Folate vs. Folic Acid: What’s the Difference?

Though often used interchangeably, folate and folic acid are distinct forms of vitamin B9:

- Folate refers to the naturally occurring form found in food, including green leafy vegetables, citrus fruits, and beans.

- Folic acid is the synthetic version of folate, commonly added to supplements and fortified foods like rice, pasta, bread, and some breakfast cereals. Research shows folic acid effectively prevents neural tube defects, making it a critical addition to prenatal care.

Types of Folate:

Folate is a general term for various forms of vitamin B9, including folic acid, dihydrofolate (DHF), tetrahydrofolate (THF), 5,10-methylenetetrahydrofolate (5,10-MTHF), and 5-methyltetrahydrofolate (5-MTHF). While supplements may contain forms like 5-MTHF, their effectiveness in preventing neural tube defects hasn’t been extensively studied, unlike folic acid.

Folate Reference Ranges (ng/mL):

  • Normal: >5.38
  • Borderline Deficient: 3.38–5.38
  • Deficient: 0.35–3.37
  • Excessive: >24.00

Maintaining adequate folate intake, whether from food, supplements, or a combination, is essential for overall health and particularly crucial during pregnancy.

What does it mean if your Vitamin B9 (Folate) result is too high?

High Folate (Serum) Levels – What Do They Mean?

Seeing a high folate level on your lab results can be confusing—but in most cases, it’s not a cause for concern.

Folate (also known as vitamin B9) is a water-soluble vitamin found in leafy greens, beans, fortified foods, and supplements. Because it’s water-soluble, your body can flush out extra amounts through urine. This means that high folate levels are usually due to a folate-rich diet or folic acid supplements—and they rarely cause harm.

What’s Considered a High Folate Level?

Most labs consider levels above 20 ng/mL (45.3 nmol/L) to be elevated, although many do not define a strict upper limit. Levels above 24–30 ng/mL may raise some flags, especially if you're not taking supplements or eating unusually high amounts of folate.

Could It Mean Something Else?

Yes. In some cases, a high folate level may actually be a sign of vitamin B12 deficiency. Your body needs vitamin B12 to properly use folate. Without enough B12, folate can build up in your blood—even though your cells can’t use it effectively. This can mask symptoms of B12 deficiency, like fatigue, brain fog, or nerve issues.

Important Note:

A high folate result does not confirm or rule out vitamin B12 deficiency. If there’s any suspicion of low B12, it’s important to test vitamin B12 directly—along with markers like methylmalonic acid (MMA) and homocysteine—for a clearer picture.

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What does it mean if your Vitamin B9 (Folate) result is too low?

A low Folate level may mean you have a problem with your diet, alcohol dependence, or an eating disorder such as anorexia nervosa. It may also mean you have a problem absorbing or using Folate, such as: vitamin C deficiency, liver disease, celiac disease, or Crohn’s disease. Signs and symptom of folate deficiency include:

- Diarrhea

- Dizziness

- Fatigue

- Loss of appetite

- Rapid or irregular heart rate

- Confusion

- Paranoia 

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