4-OH-E1 (male)

A healthy result should fall into the range 0 - 0.8 ng/mg.

4-OH-E1 is referred to as the “bad” estrogen, along with 16-OH-E1.

  • It is a minor pathway of estrogen metabolism.
  • It may also enhance cancer development.
  • It may directly damage DNA by causing breaks in the molecular strands of DNA. 

4-Hydroxyestrones have the ability to convert to metabolites that react with DNA and cause mutations that can be carcinogenic. It is also present in greater quantities in patients deficient in methionine and folic acid. 


  • Spink BC, Fasco MJ, Gierthy JF, Spink DC. 12-O-tetradecanoylphorbol-13-acetate upregulates the Ah receptor and differentially alters CYP1B1 and CYP1A1 expression in MCF-7 breast cancer cells. J Cell Biochem. Sep 1 1998;70(3):289- 296.
  • Hayes CL, Spink DC, Spink BC, Cao JQ, Walker NJ, Sutter TR. 17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1. Proc Natl Acad Sci USA. Sep 3 1996;93(18):9776-9781.
  • Yang L, Gaikwad NW, Meza J, et al. Novel biomarkers for risk of prostate cancer: results from a case-control study. Prostate. Jan 1 2009;69(1):41-48.
  • Castagnetta LA, Granata OM, Traina A, et al. Tissue content of hydroxyestrogens in relation to survival of breast cancer patients. Clin Cancer Res. Oct 2002;8(10):3146-3155.
  • https://www.sciencedaily.com/releases/2010/04/100419150813.htm

4-OH-E1 (male) result calculator

insert the value from you 4-OH-E1 (male) test result.

What does it mean if your 4-OH-E1 (male) result is too low?

Generally no treatment recommended

What does it mean if your 4-OH-E1 (male) result is too high?

This particular estrogen metabolite appears to be more abundant among men diagnosed with prostate cancer [1]

Possible treatment (please discuss with your doctor before starting any type of treatment)

  • Improve methylation by adding cofactors (B12, folate) or methyl donors (betaine, dimethyl glycine)
  • Consider genetic testing for COMT and CYP1B1 activity, particularly if positive family history
  • Reduce stress: COMT is involved in the metabolism of epinephrine, reducing availability for estrogen metabolism
  • Increase inhibitors of CYP1B1
    • Grapefruit
    • Ginseng
  • Avoidance of CYP1B1 inducers
    • Polycyclic aromatic hydrocarbons
  • Evaluate methylation activity:
    • Serum Homocysteine
    • Serum B12 or methylation
    • Urinary FIGLU
    • Urinary xanthurenate
  • Other potential protective factors:
    • Glutathione (reduction of estrogen quinones)
    • Resveratrol (prevents estrogen quinone formation)
    • Selenium, zinc, magnesium

Are you concerned about your lab report?
Select your plan, upload your lab reports and we’ll provide you with a meaningful interpretation.

Basic Interpretation
  • Instant interpretation
  • Use self upload form
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Cancel anytime
Full Interpretation
  • Instant interpretation
  • We upload 4 reports for you
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Cancel anytime
  • Online support
Own It For Life
  • Unlimited access
  • No subscription
  • Instant interpretations
  • We upload 10 reports for you
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Dedicated support

Test results don't lie. Learn what is happening inside your body.

Health is not exactly rocket science. You just have to know what your body needs and doesn't need. Your medical tests contain that information. Upload them to our service and we’ll help you understand, organize, and act on them.

Get Started

Medical results explained and interpreted.


Healthmatters.io customer review

"I was really concerned about my lab results and my GP was not able to see me for another week. Through HealthMatters I was able to interpret my results instantly."

Healthmatters.io thankfull female customer

"I've been keeping all my lab results in a spreadsheet, covering approximately 20 years. The HealthMatters team helped me upload that info into the cloud. I can show this data to my family and doctors now easily."

Healthmatters.io thankful young female customer

"I had no idea what my lab results meant. Here I found all the biomarkers that I was looking for online. Finally, I have not only organized them all neatly in one place, but I am also grateful to be able to understand what they mean."

Privacy and security of personal data. HIPAA and GDPR compliant badges.

Your Privacy And Security

At HealthMatters, we're committed to maintaining the security and confidentiality of your personal information. We've put security measures in place to help protect against the loss, misuse or alteration of information under our control. We use procedural, physical and electronic security methods designed to prevent people who aren't authorized from getting access to this information. Our internal code of conduct adds additional privacy protection. See our Privacy Policy for more information.