2-OH-E1 (male)

A healthy result should fall into the range 0 - 5.9 ng/mg.

2-OH-E1 is considered a ‘good estrogen’ and it does not stimulate cell growth. It can block the action of stronger, potentially carcinogenic estrogens. When it is methylated to 2-Me-E1 it is cancer protective.

2-Hydroxyestrone is an endogenous biomarker and major urinary metabolite of estrone and estradiol. Along with 16α-Hydroxyestrone, 2-Hydroxyestrone is used as an indicator for increased risk of breast cancer. 

This metabolite of Estrone is considered protective. A comparison with 2-Methoxyestrone, its Phase II liver metabolite, may help with assessing adequacy of methylation processes.

The 2:16alpha-Hydroxyestrone ratio appears from the literature to be an important gauge of estrogen metabolism. In general, the higher the ratio, the less association there is with estrogen-dependent diseases such as breast cancer and lupus, and the more likely the person has a beneficial hormone metabolism. A 2:16alpha-hydroxyestrone ratio in serum greater than 0.4 is generally thought to be beneficial. There are numerous modifiers of this value, most of which induce changes in the level of 2-OHE1. These include intake of indole-3-carbinols from cruciferous vegetables, flaxseed, soy, omega-3 fatty acids, and vigorous exercise. All are shown to improve the levels of 2-OHE1 in most individuals. It is to be emphasized that some individuals in clinical studies have exhibited a paradoxical response to treatments that would typically raise the 2-OHE1 levels. Therefore, follow-up testing after treatment is strongly suggested. There may be an increased likelihood of osteoporosis with excessive 2-OHE1 production. It is important to note that the ideal upper limit of 2-OHE1 is not apparent from the existing literature. Attention to bone loss processes in the urine is perhaps warranted in individuals with a very high 2:16alpha-hydroxyestrone ratio.


  • https://www.ncbi.nlm.nih.gov/pubmed/16289388
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766371
  • www.ncbi.nlm.nih.gov/pubmed/11991791


2-OH-E1 (male) result calculator

insert the value from you 2-OH-E1 (male) test result.

What does it mean if your 2-OH-E1 (male) result is too high?

High levels of the “good estrogen” are typically considered beneficial; however, when coupled with a low 2-OMe-E1, it may indicate poor methylation activity.

If both 2-OH-E1 and 16a-OHE1 are high, then total estrogen load may be high. Consider methods to better clear the estrogens from the body.

Are you concerned about your lab report?
Select your plan, upload your lab reports and we’ll provide you with a meaningful interpretation.

Basic Interpretation
  • Instant interpretation
  • Use self upload form
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Cancel anytime
Full Interpretation
  • Instant interpretation
  • We upload 4 reports for you
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Cancel anytime
  • Online support
Own It For Life
  • Unlimited access
  • No subscription
  • Instant interpretations
  • We upload 10 reports for you
  • 800+ biomarkers explained
  • Nutrition Recommendations
  • Dedicated support

Test results don't lie. Learn what is happening inside your body.

Health is not exactly rocket science. You just have to know what your body needs and doesn't need. Your medical tests contain that information. Upload them to our service and we’ll help you understand, organize, and act on them.

Get Started

Medical results explained and interpreted.


Healthmatters.io customer review

"I was really concerned about my lab results and my GP was not able to see me for another week. Through HealthMatters I was able to interpret my results instantly."

Healthmatters.io thankfull female customer

"I've been keeping all my lab results in a spreadsheet, covering approximately 20 years. The HealthMatters team helped me upload that info into the cloud. I can show this data to my family and doctors now easily."

Healthmatters.io thankful young female customer

"I had no idea what my lab results meant. Here I found all the biomarkers that I was looking for online. Finally, I have not only organized them all neatly in one place, but I am also grateful to be able to understand what they mean."

Privacy and security of personal data. HIPAA and GDPR compliant badges.

Your Privacy And Security

At HealthMatters, we're committed to maintaining the security and confidentiality of your personal information. We've put security measures in place to help protect against the loss, misuse or alteration of information under our control. We use procedural, physical and electronic security methods designed to prevent people who aren't authorized from getting access to this information. Our internal code of conduct adds additional privacy protection. See our Privacy Policy for more information.