Genova Diagnostics

One Day Hormone Check

21 biomarkers Sample: Saliva Peer-reviewed

One Day Hormone Check: Comprehensive Salivary Hormone Testing

The One Day Hormone Check is a non-invasive salivary hormone test designed to assess bioavailable, unbound hormone levels over a 24-hour period. This test provides insights into key hormones, including:

- Estrogen markers – Estradiol, Estrone, Estriol
- Progesterone & the P/E2 ratio (Progesterone-to-Estradiol)
- Testosterone
- Cortisol (stress hormone)
- DHEA (precursor to sex hormones)
- Melatonin (sleep-regulating hormone)

Who Can Benefit from the One Day Hormone Check?

This test helps identify hormonal imbalances that impact both men and women. It is particularly useful for:

Men experiencing symptoms of andropause (male menopause)
Women navigating perimenopause or menopause
Individuals with adrenal dysfunction, chronic stress, or sleep disturbances

By analyzing hormone interactions—such as sex hormones, adrenal function (DHEA & cortisol), and sleep-wake cycles (melatonin)—clinicians can personalize hormone replacement therapy (HRT), nutritional interventions, and lifestyle recommendations.

Signs of Hormonal Imbalance:

People with hormonal imbalances may experience:

Fatigue & low energy
Unexplained weight gain or loss
Menstrual irregularities (women)
Decreased libido & sexual dysfunction
Insomnia or poor sleep quality
Changes in hair, skin, or mood
Hot flashes & night sweats

Understanding Reference Ranges

The therapeutic reference ranges provided are based on a cohort of perimenopausal and menopausal women (ages 37-62) who were treated with bioidentical hormone therapy (HT). This therapy included combinations of:

Biest (transdermal estradiol + estriol)
Oral micronized progesterone
Transdermal testosterone
Oral 7-keto-DHEA

Note: These ranges are for informational purposes only. Individual hormone levels should be interpreted in the context of personal health history and clinical symptoms.

Biomarkers in this category

21 biomarkers · optimal ranges shown

Cortisol Awakening Response, Percent Increase

Saliva

CAR

DHEA (7AM - 9AM)

Produced by both the adrenal gland and the brain

DHEA: Cortisol Ratio/10,000

The DHEA: Cortisol Ratio is an important marker that helps assess the balance between two crucial hormones produced by the adrenal glands: dehydroepiandrosterone (DHEA) and cortisol. DHEA is often referred to as a "youth hormone" because it supports immune function, mood, energy levels, and overall well-being. On the other hand, cortisol is known as the "stress hormone" because it plays a key role in the body's response to stress, affecting metabolism, immune response, and energy levels. The ratio between these two hormones is significant because it provides insight into how well the adrenal glands are functioning. A balanced ratio indicates that the body is managing stress well and maintaining overall hormonal balance. However, an imbalance, such as a high cortisol level with low DHEA, can indicate chronic stress, adrenal fatigue, or other health issues. Conversely, a high DHEA level with low cortisol might suggest different imbalances or conditions. By evaluating the DHEA: Cortisol Ratio, healthcare providers can better understand an individual's stress response and overall adrenal health, allowing them to recommend targeted interventions such as lifestyle changes, stress management techniques, or specific supplements to restore balance and improve health outcomes.

DHEA:Cortisol Ratio

This calculation represents anabolic and catabolic balance

Estradiol (E2)

important female hormone

Estriol (E3)

important female hormone

Estrone (E1)

important female hormone

P/E2 Ratio

relationship between progesterone and estradiol levels

Progesterone

important for normal reproductive and menstrual function