IL-5
Interleukin-5 (IL-5) is an anti-inflammatory cytokine primarily associated with T-helper 2 (Th2) immune responses. It plays a central role in the growth, activation, and survival of eosinophils—a type of white blood cell involved in allergic reactions, asthma, and defense against parasites. Produced mainly by Th2 cells, mast cells, and type 2 innate lymphoid cells (ILC2s), IL-5 acts as a key messenger that connects immune regulation with allergic inflammation and mucosal immunity.
Biological Function
IL-5’s primary functions revolve around eosinophil regulation and antibody production. It helps maintain a balanced immune defense by:
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Stimulating the growth and activation of eosinophils in the bone marrow and bloodstream
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Prolonging eosinophil survival to support parasite defense and allergic responses
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Enhancing B-cell maturation and immunoglobulin (IgA and IgE) production
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Supporting mucosal barrier integrity, especially in respiratory and gastrointestinal tissues
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Modulating inflammation to limit tissue injury after infection or immune activation
Through these actions, IL-5 helps coordinate immune defense against parasites and contributes to Th2-dominant responses that counterbalance Th1-driven inflammation.
Elevated IL-5 Levels
High IL-5 levels indicate activation of the Th2 immune pathway, often in response to allergens or parasites. Elevated IL-5 increases eosinophil production and activity, which can lead to eosinophilic inflammation—a hallmark of many allergic and respiratory diseases.
Common associations with elevated IL-5 include:
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Allergic disorders, such as asthma, atopic dermatitis, eczema, and allergic rhinitis
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Eosinophilic inflammation, including eosinophilic esophagitis (EoE) and hypereosinophilic syndromes
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Parasitic infections, particularly helminthic (worm) infections
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Chronic inflammatory airway diseases, where IL-5 contributes to airway hyperreactivity and mucus production
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Certain autoimmune conditions, where inappropriate Th2 activation drives tissue inflammation
Persistently elevated IL-5 can result in tissue damage, airway remodeling, and chronic allergic inflammation due to prolonged eosinophil activation.
Low IL-5 Levels
Low IL-5 levels may reflect a Th1- or Th17-dominant immune profile, where inflammatory responses overshadow antibody and eosinophil-mediated defense. This can result in reduced mucosal protection and increased vulnerability to infections affecting the skin, gut, or respiratory tract. Low IL-5 may also appear in individuals undergoing immunosuppressive therapy or experiencing immune exhaustion.
IL-5 in the Cytokine Response Profile (CytoDx)
Within the Cytokine Response Profile (CytoDx) by Diagnostic Solutions Laboratory, IL-5 is grouped among the anti-inflammatory (Th2) cytokines, alongside IL-4, IL-10, and IL-13. Together, these markers reveal how effectively the immune system regulates inflammation and maintains balance between cell-mediated (Th1) and antibody-mediated (Th2) responses.
A high IL-5 reading in this panel typically suggests Th2 dominance—a skew toward allergic or atopic immune activity—while low IL-5 may indicate Th1/Th17 dominance, reflecting more pro-inflammatory immune bias.
Clinical Insights
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High IL-5: Suggests Th2-driven immune activity; commonly linked to allergies, asthma, or eosinophilic inflammation.
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Low IL-5: Reflects reduced Th2 signaling or anti-inflammatory capacity, often seen in chronic inflammation or autoimmunity.
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Balanced IL-5: Indicates healthy Th1/Th2 regulation and optimal mucosal immune defense.
Supporting Healthy IL-5 Balance
Balancing IL-5 activity involves reducing chronic inflammation and supporting immune regulation through lifestyle and clinical strategies such as:
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Managing allergies and asthma triggers, including environmental and dietary allergens
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Anti-inflammatory and antioxidant nutrition, including omega-3 fatty acids, quercetin, and vitamin D
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Gut microbiome support, as dysbiosis can increase Th2 activation and allergic sensitivity
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Stress reduction and adequate sleep, both essential for cytokine balance and immune homeostasis
Summary
IL-5 (Interleukin-5) is a Th2-associated cytokine that regulates eosinophil activity and supports antibody-mediated immunity. Elevated IL-5 is commonly linked to allergic conditions, asthma, and parasitic infections, while low levels may reflect impaired anti-inflammatory or mucosal defense. Within the Cytokine Response Profile (CytoDx) by Diagnostic Solutions Laboratory, IL-5 helps reveal whether immune activity is skewed toward Th2-driven responses or inflammatory Th1/Th17 dominance, offering valuable insight into immune regulation and chronic inflammatory patterns.
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