The beta-amyloid 42/40 ratio (Aβ42/40) compares two forms of amyloid-beta protein found in blood (plasma/serum) or cerebrospinal fluid (CSF): Aβ42 (a 42–amino-acid peptide) and Aβ40 (40–amino-acid peptide). In Alzheimer’s disease (AD), Aβ42 is more prone to aggregate into plaques in the brain—so its measured level decreases in fluids relative to Aβ40. A lower Aβ42/40 ratio therefore increases the likelihood of underlying amyloid plaque pathology consistent with AD biology.
Why it matters
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Early, less-invasive insight: Blood Aβ42/40 can help identify amyloid changes earlier in the diagnostic workup and may reduce the need for PET imaging or lumbar puncture in many patients.
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Better signal than Aβ42 alone: Normalizing Aβ42 to Aβ40 reduces person-to-person variability, improving accuracy over Aβ42 by itself.
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Guides next steps: When combined with phospho-tau markers (e.g., p-tau217 or p-tau181), the ratio helps clinicians decide who should proceed to confirmatory testing and informs treatment discussions.
How it’s measured
Labs use immunoassays or mass spectrometry in plasma/serum or CSF. Each platform has its own reference intervals and cutoffs. Results are typically reported as a unitless ratio (Aβ42 divided by Aβ40). Because assays are not interchangeable, interpretation must follow the cutoff printed on your report.
When this test is useful
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New or progressive memory and thinking changes under evaluation
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Distinguishing Alzheimer’s disease from other causes of cognitive impairment
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Triage before more invasive/expensive tests (amyloid PET, CSF biomarkers)
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Establishing a baseline before considering disease-modifying therapies
How to interpret your result
Always rely on the lab’s stated cutoff/interval. Do not compare numbers across different labs or methods.
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Low Aβ42/40 ratio (below the lab’s cutoff): Raises the likelihood of amyloid plaque pathology consistent with AD. Your clinician may recommend confirmatory testing (amyloid PET or CSF) if results will change management.
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Normal/High Aβ42/40 ratio (at or above cutoff): Makes AD-type amyloid pathology less likely at the time of testing; your care team may evaluate other causes of symptoms.
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Borderline/indeterminate: Some labs define a gray zone. In that case, your clinician may suggest repeat testing, combining with p-tau blood tests, or proceeding to confirmatory studies based on overall risk.
Practical reading tips
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Pair the ratio with phosphorylated-tau (e.g., p-tau217 or p-tau181) for higher diagnostic confidence.
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Consider clinical context: symptom history, cognitive testing, MRI or other imaging, and routine labs to rule out reversible contributors.
What can affect the number?
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Assay & platform differences: Methods (immunoassay vs mass spec) yield different numeric ranges—cutoffs are assay-specific.
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Preanalytical variables: Fasting status, tube type, processing time, and storage can influence measurements; labs standardize these steps to reduce variability.
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Biological factors: Acute illness, severe kidney/liver disease, and mixed neurodegenerative pathologies can complicate interpretation.
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Population & setting: Performance is strongest in symptomatic adults assessed in specialty memory clinics; routine screening of asymptomatic individuals is not established.
Related biomarkers often ordered together
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p-tau217 or p-tau181 (blood/CSF): Improves rule-in/rule-out accuracy when combined with Aβ42/40.
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Aβ42/40 in CSF and CSF p-tau/total tau: Long-standing core CSF biomarkers in AD.
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NfL (neurofilament light): Marker of neurodegeneration (not AD-specific).
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MRI, FDG-PET, amyloid PET, cognitive testing: Provide structural/functional context.
Limitations & caveats
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Not a diagnosis by itself. The ratio indicates the likelihood of amyloid pathology and must be interpreted with clinical evaluation and, when needed, confirmatory tests.
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Gray-zone results happen. A minority of patients fall into an indeterminate range; management depends on symptoms and pretest probability.
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Changing results over time. If symptoms evolve or initial findings are borderline, re-testing can be helpful.
FAQs
Is there a single “normal” value for Aβ42/40?
No. Ranges and cutoffs are lab- and method-specific. Always use the interval printed on your report.
Which is better—blood or CSF?
CSF is highly validated but invasive. Blood offers convenience and growing evidence; many pathways now use blood Aβ42/40 plus p-tau to triage for PET/CSF.
Do medications or supplements change the result?
Most common medicines don’t meaningfully alter the ratio, but acute illness and major organ dysfunction can affect interpretation. Discuss your full medication list and health status with your clinician.
If my ratio is low, do I automatically have Alzheimer’s?
No. A low ratio increases the likelihood of AD-type amyloid pathology; diagnosis still requires clinical assessment (and sometimes PET or CSF confirmation).
What to do next
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Read your report’s cutoff and note the assay/platform name.
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Discuss context with your clinician: symptoms, family history, cognitive testing results.
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Consider companion tests (e.g., p-tau217, p-tau181) to strengthen interpretation.
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Plan follow-up: repeat testing or confirmatory PET/CSF if results are borderline or management would change.
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Track over time if symptoms progress or treatment is started.
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